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http://hdl.handle.net/2445/44837
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DC Field | Value | Language |
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dc.contributor.author | Ortega González, Fco. Javier | - |
dc.contributor.author | Jolkkonen, Jukka | - |
dc.contributor.author | Mahy Gehenne, Josette Nicole | - |
dc.contributor.author | Rodríguez Allué, Manuel José | - |
dc.date.accessioned | 2013-07-16T11:05:33Z | - |
dc.date.available | 2014-05-14T22:02:11Z | - |
dc.date.issued | 2012-11-14 | - |
dc.identifier.issn | 0271-678X | - |
dc.identifier.uri | http://hdl.handle.net/2445/44837 | - |
dc.description.abstract | Glibenclamide is neuroprotective against cerebral ischemia in rats. We studied whether glibenclamide enhances long-term brain repair and improves behavioral recovery after stroke. Adult male Wistar rats were subjected to transient middle cerebral artery occlusion (MCAO) for 90 minutes. A low dose of glibenclamide (total 0.6mg) was administered intravenously 6, 12, and 24 hours after reperfusion. We assessed behavioral outcome during a 30-day follow-up and animals were perfused for histological evaluation. In vitro specific binding of glibenclamide to microglia increased after pro-inflammatory stimuli. In vivo glibenclamide was associated with increased migration of doublecortin-positive cells in the striatum toward the ischemic lesion 72 hours after MCAO, and reactive microglia expressed sulfonylurea receptor 1 (SUR1) and Kir6.2 in the medial striatum. One month after MCAO, glibenclamide was also associated with increased number of NeuN-positive and 5-bromo-2-deoxyuridine-positive neurons in the cortex and hippocampus, and enhanced angiogenesis in the hippocampus. Consequently, glibenclamide-treated MCAO rats showed improved performance in the limb-placing test on postoperative days 22 to 29, and in the cylinder and water-maze test on postoperative day 29. Therefore, acute blockade of SUR1 by glibenclamide enhanced long-term brain repair in MCAO rats, which was associated with improved behavioral outcome. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | International Society for Cerebral Blood Flow and Metabolism | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1038/jcbfm.2012.166 | - |
dc.relation.ispartof | Journal of Cerebral Blood Flow and Metabolism, 2013, vol. 33, num. 3, p. 356-364 | - |
dc.relation.uri | http://dx.doi.org/10.1038/jcbfm.2012.166 | - |
dc.rights | (c) Ortega González, Fco. Javier et al., 2013 | - |
dc.source | Articles publicats en revistes (Ciències Fisiològiques) | - |
dc.subject.classification | Isquèmia cerebral | - |
dc.subject.classification | Micròglia | - |
dc.subject.classification | Regeneració del sistema nerviós | - |
dc.subject.other | Cerebral ischemia | - |
dc.subject.other | Microglia | - |
dc.subject.other | Nervous system regeneration | - |
dc.title | Glibenclamide enhances neurogenesis and improves long-term functional recovery after transient focal cerebral ischemia | eng |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 617153 | - |
dc.date.updated | 2013-07-16T10:05:45Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 23149556 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) |
Files in This Item:
File | Description | Size | Format | |
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617153.pdf | 1.67 MB | Adobe PDF | View/Open |
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