Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/46331
Title: | Neurotoxicity of prion peptides mimicking the central domain of the cellular prion protein |
Author: | Vilches Saez, Silvia Vergara Paños, Cristina Nicolás i Pallejà, Josep Oriol Sanclimens Pérez de Rozas, Glòria Varón, S. Acosta, Gerardo A. Albericio Palomera, Fernando Royo Expósito, Miriam Río Fernández, José Antonio del Gavín Marín, Rosalina |
Keywords: | Prions Pèptids Proteïnes Síntesi de pèptids Mort cel·lular Prions Peptides Proteins Peptide synthesis Cell death |
Issue Date: | 5-Aug-2013 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | The physiological functions of PrP(C) remain enigmatic, but the central domain, comprising highly conserved regions of the protein may play an important role. Indeed, a large number of studies indicate that synthetic peptides containing residues 106-126 (CR) located in the central domain (CD, 95-133) of PrP(C) are neurotoxic. The central domain comprises two chemically distinct subdomains, the charge cluster (CC, 95-110) and a hydrophobic region (HR, 112-133). The aim of the present study was to establish the individual cytotoxicity of CC, HR and CD. Our results show that only the CD peptide is neurotoxic. Biochemical, Transmission Electron Microscopy and Atomic Force Microscopy experiments demonstrated that the CD peptide is able to activate caspase-3 and disrupt the cell membrane, leading to cell death. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0070881 |
It is part of: | PLoS One, 2013, vol. 8, num. 8, p. e70881 |
URI: | http://hdl.handle.net/2445/46331 |
Related resource: | http://dx.doi.org/10.1371/journal.pone.0070881 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
628364.pdf | 6 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License