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http://hdl.handle.net/2445/49465
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DC Field | Value | Language |
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dc.contributor.author | Sánchez-Moya, Albert | - |
dc.contributor.author | Pedroso Muller, Enrique | - |
dc.contributor.author | Grandas Sagarra, Anna | - |
dc.date.accessioned | 2014-02-06T10:17:05Z | - |
dc.date.available | 2014-02-06T10:17:05Z | - |
dc.date.issued | 2012-01-13 | - |
dc.identifier.issn | 1043-1802 | - |
dc.identifier.uri | http://hdl.handle.net/2445/49465 | - |
dc.description.abstract | Phosphorothioate diester oligonucleotides proved to be fully compatible with maleimides in the context of two different conjugation reactions: (a) reaction of 5′diene-[phosphorothioate oligonucleotides] with maleimido-containing compounds to afford the Diels-Alder cycloadduct; (b) conjugation of 5′maleimido-[phosphorothioate oligonucleotides] with thiol-containing compounds. No evidence of reaction between phosphorothioate diesters and maleimides was found in any of these processes. Importantly, in the preparation of 5′maleimido-[phosphorothioate oligonucleotides] from [protected maleimido]-[phosphorothioate oligonucleotides], which requires the maleimide to be deprotected by retro-Diels-Alder reaction (heating for 3-4 h in toluene at 90 °C), no addition of phosphorothioate diester to the maleimide was found either. Finally, maleimide-[phosphorothioate monoester] conjugation was also explored for comparison purposes. | - |
dc.format.extent | 24 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Chemical Society | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1021/bc200598g | - |
dc.relation.ispartof | Bioconjugate Chemistry, 2012, vol. 23, num. 2, p. 300-307 | - |
dc.relation.uri | http://dx.doi.org/10.1021/bc200598g | - |
dc.rights | (c) American Chemical Society , 2012 | - |
dc.source | Articles publicats en revistes (Química Inorgànica i Orgànica) | - |
dc.subject.classification | Oligonucleòtids | - |
dc.subject.classification | Immunologia | - |
dc.subject.classification | Àcids nucleics | - |
dc.subject.classification | Bioquímica | - |
dc.subject.classification | Proteïnes | - |
dc.subject.other | Oligonucleotides | - |
dc.subject.other | Immunology | - |
dc.subject.other | Nucleic acids | - |
dc.subject.other | Biochemistry | - |
dc.subject.other | Proteins | - |
dc.title | Conjugation reactions involving maleimides and phosphorothioate oligonucleotides | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 605970 | - |
dc.date.updated | 2014-02-06T10:17:05Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 22243598 | - |
Appears in Collections: | Articles publicats en revistes (Química Inorgànica i Orgànica) |
Files in This Item:
File | Description | Size | Format | |
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605970.pdf | 767.41 kB | Adobe PDF | View/Open |
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