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Title: | Self-Renewing Human Bone Marrow Mesenspheres Promote Hematopoietic Stem Cell Expansion. |
Author: | Isern, Joan Martín-Antonio, Beatriz Ghazanfari, Roshanak Martín, Aan M. López, Juan A. Toro, Raquel del Sánchez-Aguilera, Abel Arranz, Lorena Martín-Pérez, Daniel Suárez-Lledó, María Marín, Pedro Van Pel, Melissa Fibbe, Willem E. Vázquez, Jesús Scheding, Stefan Urbano Ispizua, Álvaro Méndez-Ferrer, Simón |
Keywords: | Diferenciació cel·lular Hematopoesi Medul·la òssia Cell diferentiation Hematopoiesis Bone marrow |
Issue Date: | 25-Apr-2013 |
Publisher: | Elsevier |
Abstract: | Strategies for expanding hematopoietic stem cells (HSCs) include coculture with cells that recapitulate their natural microenvironment, such as bone marrow stromal stem/progenitor cells (BMSCs). Plastic-adherent BMSCs may be insufficient to preserve primitive HSCs. Here, we describe a method of isolating and culturing human BMSCs as nonadherent mesenchymal spheres. Human mesenspheres were derived from CD45- CD31- CD71- CD146+ CD105+ nestin+ cells but could also be simply grown from fetal and adult BM CD45--enriched cells. Human mesenspheres robustly differentiated into mesenchymal lineages. In culture conditions where they displayed a relatively undifferentiated phenotype, with decreased adherence to plastic and increased self-renewal, they promoted enhanced expansion of cord blood CD34+ cells through secreted soluble factors. Expanded HSCs were serially transplantable in immunodeficient mice and significantly increased long-term human hematopoietic engraftment. These results pave the way for culture techniques that preserve the self-renewal of human BMSCs and their ability to support functional HSCs. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1016/j.celrep.2013.03.041 |
It is part of: | Cell Reports, 2013, vol. 3, num. 5, p. 1714-1724 |
URI: | http://hdl.handle.net/2445/51084 |
Related resource: | http://dx.doi.org/10.1016/j.celrep.2013.03.041 |
ISSN: | 2211-1247 |
Appears in Collections: | Articles publicats en revistes (Medicina) |
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