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http://hdl.handle.net/2445/52724
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DC Field | Value | Language |
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dc.contributor.author | Noguera, Aina | - |
dc.contributor.author | Gomez, Cristina | - |
dc.contributor.author | Faner, Rosa | - |
dc.contributor.author | Cosío, Borja G. | - |
dc.contributor.author | González Périz, Ana | - |
dc.contributor.author | Clària i Enrich, Joan | - |
dc.contributor.author | Carvajal, Angel | - |
dc.contributor.author | Agustí García-Navarro, Àlvar | - |
dc.date.accessioned | 2014-03-21T08:39:09Z | - |
dc.date.available | 2014-03-21T08:39:09Z | - |
dc.date.issued | 2012-11-13 | - |
dc.identifier.issn | 1465-9921 | - |
dc.identifier.uri | http://hdl.handle.net/2445/52724 | - |
dc.description.abstract | Background: Chronic Obstructive Pulmonary Disease (COPD) is characterized by an enhanced inflammatory response to smoking that persists despite quitting. The resolution of inflammation (catabasis) is a complex and highly regulated process where tissue resident macrophages play a key role since they phagocytose apoptotic cells (efferocytosis), preventing their secondary necrosis and the spill-over of their pro-inflammatory cytoplasmic content, and release pro-resolution and tissue repair molecules, such as TGFβ, VEGF and HGF. Because inflammation does not resolve in COPD, we hypothesized that catabasis may be abnormal in these patients. Methods: To explore this hypothesis, we studied lung tissue samples obtained at surgery from 21 COPD patients,22 smokers with normal spirometry and 13 non-smokers controls. In these samples we used: (1)immunohistochemistry to assess the expression of CD44, CD36, VEGF and TGFβ in lung macrophages; (2) real time PCR to determine HGF, PPARγ, TGFβ, VEGF and MMP-9 gene expression; and, (3) ELISA to quantify lipoxin A4, a lipid mediator of catabasis. Results: We found that current and former smokers with COPD showed: (1) more inflammation (higher MMP-9 expression); (2) reduced macrophage surface expression of CD44, a key efferocytosis receptor; and, (3) similar levels of TGFβ, VEGF, HGF, PPARγ, and lipoxin A4 than smokers with normal spirometry, despite the presence of inflammation and disease. Conclusions: These results identify several potential abnormalities of catabasis in patients with COPD. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1186/1465-9921-13-101 | - |
dc.relation.ispartof | Respiratory Research, 2012, vol. 13, p. 101 | - |
dc.relation.uri | http://dx.doi.org/10.1186/1465-9921-13-101 | - |
dc.rights | cc-by (c) Noguera, A. et al., 2012 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Medicina) | - |
dc.subject.classification | Malalties pulmonars obstructives cròniques | - |
dc.subject.classification | Bronquitis | - |
dc.subject.classification | Immunologia | - |
dc.subject.other | Chronic obstructive pulmonary diseases | - |
dc.subject.other | Bronchitis | - |
dc.subject.other | Immunology | - |
dc.title | An investigation of the resolution of inflammation (catabasis) in COPD. | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 625715 | - |
dc.date.updated | 2014-03-21T08:39:09Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 23148928 | - |
Appears in Collections: | Articles publicats en revistes (Medicina) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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625715.pdf | 1.22 MB | Adobe PDF | View/Open |
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