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Title: | Enhancing hepatic fatty acid oxidation as a strategy for reversing metabolic disorders programmed by maternal undernutrition during gestation |
Author: | Torrens, Juana María Orellana Gavaldà, Josep Maria Palou, Mariona Sánchez, Juana Herrero Rodríguez, Laura Picó, Catalina Serra i Cucurull, Dolors Palou Oliver, Andreu |
Keywords: | Àcids grassos Dieta hipocalòrica Fetge Teràpia genètica Malnutrició Complicacions en l'embaràs Trastorns del metabolisme Fatty acids Low-calorie diet Liver Gene therapy Malnutrition Disorders of metabolism Complications of pregnancy |
Issue Date: | May-2014 |
Publisher: | Karger |
Abstract: | Background/Aims: Moderate maternal calorie-restriction during gestation programmes offspring for a major propensity to develop metabolic alterations in adulthood. We aimed to assess whether increased hepatic fatty-acid oxidation (FAO), at early ages, by gene transfer of Cpt1am (active mutant of carnitine palmitoyltransferase-1a), may be a strategy for reversing metabolic disturbances associated to maternal calorie-restriction during gestation in rats. Methods: AAV-Gfp (control) and AAV-Cpt1am vectors were administered by tail vein injection in 18-day-old control-pups and the offspring of 20% calorie-restricted rats during gestation (CR). After weaning, animals were fed with normal-fat diet. At the age of 4 months, they were moved to HF-diet and sacrificed at the age of 6 months to collect tissues. Locomotive activity, energy expenditure and blood pressure were measured. Results: Under HF-diet, CR-animals showed higher HOMA-IR, adipocyte diameter and hepatic triglyceride accumulation than controls; these alterations were reverted in Cpt1am-injected animals. In liver, this treatment ameliorated inflammatory state, decreased expression of lipogenesis-related genes and partially restored the decreased expression of leptin-receptor occurring in CR-animals. Treatment also reverted the decreased energy expenditure and the increased blood pressure of CR-animals. Conclusion: Increasing hepatic FAO through AAV-Cpt1am injection at juvenile ages prevents some metabolic disorders associated to gestational maternal calorie-restriction. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1159/000358714 |
It is part of: | Cellular Physiology and Biochemistry, 2014, vol. 33, num. 5, p. 1498-1515 |
URI: | http://hdl.handle.net/2445/59013 |
Related resource: | http://dx.doi.org/10.1159/000358714 |
ISSN: | 1015-8987 |
Appears in Collections: | Articles publicats en revistes (Institut de Biomedicina (IBUB)) Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
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