Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/66417
Title: | Plant hormones increase efficiency of reprogramming mouse somatic cells to induced pluripotent stem cells and reduce tumorigenicity |
Author: | Álvarez Palomo, Ana Belén McLenachan, Samuel Requena Osete, Jordi Menchón Najas, Cristina Barrot i Feixat, Carme Chen, Fred K. Munné Bosch, Sergi Edel, Michael John |
Keywords: | Hormones vegetals Cèl·lules mare embrionàries Citoquines Tumors Ratolins (Animals de laboratori) Plant hormones Embryonic stem cells Cytokines Tumors Mice (Laboratory animals) |
Issue Date: | 7-Jan-2014 |
Publisher: | Mary Ann Liebert, Inc. |
Abstract: | Reprogramming of somatic cells into induced pluripotent stem (iPS) cells by defined pluripotency and self-renewal factors has taken stem cell technology to the forefront of regenerative medicine. However, a number of challenges remain in the field including efficient protocols and the threat of cancer. Reprogramming of plant somatic cells to plant embryonic stem cells using a combination of two plant hormones was discovered in 1957 and has been a routine university laboratory practical for over 30 years. The plant hormones responsible for cell reprogramming to pluripotency, indole-3-acetic acid (IAA) and isopentenyl adenosine (IPA), are present in human cells, leading to the exciting possibility that plant hormones might reprogram mammalian cells without genetic factors. We found that plant hormones on their own could not reprogram mammalian cells but increase the efficiency of the early formation of iPS cells combined with three defined genetic factors during the first 3 weeks of reprogramming by accelerating the cell cycle and regulating pluripotency genes. Moreover, the cytokinin IPA, a known human anticancer agent, reduced the threat of cancer of iPS cell in vitro by regulating key cancer and stem cell-related genes, most notably c-Myc and Igf-1. In conclusion, the plant hormones, auxin and cytokinin, are new small chemicals useful for enhancing early reprogramming efficiency of mammalian cells and reducing the threat of cancer from iPS cells. These findings suggest a novel role for plant hormones in the biology of mammalian cell plasticity |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1089/scd.2013.0292 |
It is part of: | Stem Cells and Development, 2014, vol. 23, num. 6, p. 586-593 |
URI: | http://hdl.handle.net/2445/66417 |
Related resource: | http://dx.doi.org/10.1089/scd.2013.0292 |
ISSN: | 1547-3287 |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
635893.pdf | 804.83 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.