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http://hdl.handle.net/2445/69246
Title: | Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries |
Author: | Mongui, Alvaro Pérez-Llanos, Francy J. Yamamoto, Marcio M. Lozano, Marcela Zambrano, Maria M. Portillo, Patricis Del Fernández Becerra, María del Carmen Restrepo, Silvia Junca, Howard |
Director/Tutor: | Del Portillo, Hernando A. |
Keywords: | Genòmica Genètica microbiana Malària Plasmodium falciparum Resistència als medicaments Efecte dels medicaments sobre els microorganismes Genomics Microbial genetics Malaria Plasmodium falciparum Drug resistance Effect of drugs on microorganisms |
Issue Date: | 4-Jun-2015 |
Publisher: | BioMed Central |
Abstract: | BACKGROUND: The chemical treatment of Plasmodium falciparum for human infections is losing efficacy each year due to the rise of resistance. One possible strategy to find novel anti-malarial drugs is to access the largest reservoir of genomic biodiversity source on earth present in metagenomes of environmental microbial communities. METHODS: A bioluminescent P. falciparum parasite was used to quickly detect shifts in viability of microcultures grown in 96-well plates. A synthetic gene encoding the Dermaseptin 4 peptide was designed and cloned under tight transcriptional control in a large metagenomic insert context (30 kb) to serve as proof-of-principle for the screening platform. RESULTS: Decrease in parasite viability consistently correlated with bioluminescence emitted from parasite microcultures, after their exposure to bacterial extracts containing a plasmid or fosmid engineered to encode the Dermaseptin 4 anti-malarial peptide. CONCLUSIONS: Here, a new technical platform to access the anti-malarial potential in microbial environmental metagenomes has been developed. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1186/s12936-015-0748-6 |
It is part of: | Malaria Journal, 2015, vol. 14, num. 233, p. 1-6 |
URI: | http://hdl.handle.net/2445/69246 |
Related resource: | http://dx.doi.org/10.1186/s12936-015-0748-6 |
ISSN: | 1475-2875 |
Appears in Collections: | Articles publicats en revistes (ISGlobal) Publicacions de projectes de recerca finançats per la UE |
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