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http://hdl.handle.net/2445/69282
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DC Field | Value | Language |
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dc.contributor.author | Pandey, Satyaprakash | - |
dc.contributor.author | Agarwala, Prachi | - |
dc.contributor.author | Jayaraj, Gopal G. | - |
dc.contributor.author | Gargallo Gómez, Raimundo | - |
dc.contributor.author | Maiti, Souvik | - |
dc.date.accessioned | 2016-02-05T12:12:09Z | - |
dc.date.available | 2016-11-10T23:01:28Z | - |
dc.date.issued | 2015-11-10 | - |
dc.identifier.issn | 0006-2960 | - |
dc.identifier.uri | http://hdl.handle.net/2445/69282 | - |
dc.description.abstract | Biological role for existence of overlapping structures in RNA is possible yet remains very less explored. G-rich tracts of RNA form G-quadruplexes while GC-rich sequences prefer stem-loop structures. Equilibrium between alternate structures within RNA may occur and influence its functionality. We tested equilibrium between G-quadruplex and stem-loop structure in RNA and its effect on biological processes using pre-miRNA as a model system. Dicer enzyme recognizes canonical stem-loop structures in pre-miRNA to produce mature miRNAs. Deviation from stem-loop leads to deregulated mature miRNA levels, providing readout of existence of alternate structure per se G-quadruplex mediated structural interference in miRNA maturation. In vitro analysis using beacon and Dicer cleavage assays indicated that mature miRNA levels depend on relative amounts of K+ and Mg2+ ions suggesting an ion-dependent structural shift. Further in cellulo studies with and without TmPyP4 (RNA G-quadruplex destabilizer) demonstrated that miRNA biogenesis is modulated by G-quadruplex-stem-loop equilibrium in a subset of pre-miRNAs. Our combined analysis thus provides evidence for formation of non-canonical G-quadruplexes in competition with canonical stem-loop structure inside the cell and its effect on miRNA maturation in a comprehensive manner. | - |
dc.format.extent | 12 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Chemical Society | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1021/acs.biochem.5b00574 | - |
dc.relation.ispartof | Biochemistry, 2015, vol. 54, num. 48, p. 7067-7078 | - |
dc.relation.uri | http://dx.doi.org/10.1021/acs.biochem.5b00574 | - |
dc.rights | (c) American Chemical Society , 2015 | - |
dc.source | Articles publicats en revistes (Enginyeria Química i Química Analítica) | - |
dc.subject.classification | RNA | - |
dc.subject.classification | Porfirines | - |
dc.subject.other | RNA | - |
dc.subject.other | Porphyrins | - |
dc.title | RNA stem-loop to G-quadruplex equilibrium controls mature miRNA production inside the cell | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 655395 | - |
dc.date.updated | 2016-02-05T12:12:10Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 26554903 | - |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
Files in This Item:
File | Description | Size | Format | |
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655395.pdf | 1.71 MB | Adobe PDF | View/Open |
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