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http://hdl.handle.net/2445/69369
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DC Field | Value | Language |
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dc.contributor.author | Selva Jové, Laura | - |
dc.contributor.author | Benmessaoud, Rachid | - |
dc.contributor.author | Lanaspa, Miguel | - |
dc.contributor.author | Jroundi, Imane | - |
dc.contributor.author | Moraleda Redecilla, Cinta | - |
dc.contributor.author | Acácio, Sozinho | - |
dc.contributor.author | Iñigo, Melania | - |
dc.contributor.author | Bastiani, Alien | - |
dc.contributor.author | Monsonís, Manuel | - |
dc.contributor.author | Pallarés Giner, Roman | - |
dc.contributor.author | Bassat Orellana, Quique | - |
dc.contributor.author | Muñoz-Almagro, Carmen | - |
dc.date.accessioned | 2016-02-10T15:17:51Z | - |
dc.date.available | 2016-02-10T15:17:51Z | - |
dc.date.issued | 2013-10-08 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/69369 | - |
dc.description | Correction https://doi.org/10.1371/journal.pone.0147678 | - |
dc.description.abstract | Background: Dried blood spot (DBS) is a reliable blood collection method for storing samples at room temperature and easily transporting them. We have previously validated a Real-Time PCR for detection of Streptococcus pneumoniae in DBS. The objective of this study was to apply this methodology for the diagnosis of S. pneumoniae and Haemophilus influenzae b (Hib) in DBS samples of children with pneumonia admitted to two hospitals in Mozambique and Morocco. Methods: Ply and wzg genes of S. pneumoniae and bexA gene of Hib, were used as targets of Real-Time PCR. 329 DBS samples of children hospitalized with clinical diagnosis of pneumonia were tested. Results: Real-Time PCR in DBS allowed for a significant increase in microbiological diagnosis of S. pneumoniae and Hib. When performing blood bacterial culture, only ten isolates of S. pneumoniae and none of Hib were detected (3·0% positivity rate, IC95% 1·4-5·5%). Real-Time PCR from DBS samples increased the detection yield by 4x fold, as 30 S. pneumoniae and 11 Hib cases were detected (12·4% positivity rate, IC95% 9·0-16·5%; P<0·001). Conclusion: Real-Time PCR applied in DBS may be a valuable tool for improving diagnosis and surveillance of pneumonia caused by S. pneumoniae or Hib in developing countries. | - |
dc.format.extent | 6 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0076970 | - |
dc.relation.ispartof | PLoS One, 2013, vol. 8, num. 10, p. e76970 | - |
dc.relation.uri | http://dx.doi.org/10.1371/journal.pone.0076970 | - |
dc.rights | cc-by (c) Selva Jové, Laura et al., 2013 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Clíniques) | - |
dc.subject.classification | Bacteris patògens | - |
dc.subject.classification | Reacció en cadena de la polimerasa | - |
dc.subject.classification | Pneumònia | - |
dc.subject.classification | Infants | - |
dc.subject.classification | Pneumococs | cat |
dc.subject.other | Pathogenic bacteria | - |
dc.subject.other | Polymerase chain reaction | - |
dc.subject.other | Pneumonia | - |
dc.subject.other | Children | - |
dc.subject.other | Streptococcus pneumonia | eng |
dc.title | Detection of Streptococcus pneumoniae and Haemophilus influenzae type B by real-time PCR from dried blood spot samples among children with pneumonia: a useful approach for developing countries | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 640999 | - |
dc.date.updated | 2016-02-10T15:17:51Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 24116190 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
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640999.pdf | 187.66 kB | Adobe PDF | View/Open | |
677824_Correction.pdf | Correction | 123.07 kB | Adobe PDF | View/Open |
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