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|Probe-specific mixed-model approach to detect copy number differences using multiplex ligation-dependent probe amplification (MLPA)
|González, Juan R.
Carrasco Jordan, Josep Lluís
Jover Armengol, Lluís de
Estivill, Xavier, 1955-
Molecular probe techniques
|Background: MLPA method is a potentially useful semi-quantitative method to detect copy number alterations in targeted regions. In this paper, we propose a method for the normalization procedure based on a non-linear mixed-model, as well as a new approach for determining the statistical significance of altered probes based on linear mixed-model. This method establishes a threshold by using different tolerance intervals that accommodates the specific random error variability observed in each test sample. Results: Through simulation studies we have shown that our proposed method outperforms two existing methods that are based on simple threshold rules or iterative regression. We have illustrated the method using a controlled MLPA assay in which targeted regions are variable in copy number in individuals suffering from different disorders such as Prader-Willi, DiGeorge or Autism showing the best performace. Conclusion: Using the proposed mixed-model, we are able to determine thresholds to decide whether a region is altered. These threholds are specific for each individual, incorporating experimental variability, resulting in improved sensitivity and specificity as the examples with real data have revealed.
|Reproducció del document publicat a http://dx.doi.org/10.1186/1471-2105-9-261
|It is part of:
|BMC Bioinformatics, 2008, vol. 9, núm. 261
|Appears in Collections:
|Articles publicats en revistes (Medicina)
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