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dc.contributor.authorHerrero Mateu, Carmencat
dc.contributor.authorMarquès Soler, Lauracat
dc.contributor.authorLloberas Cavero, Jorgecat
dc.contributor.authorCelada Cotarelo, Antoniocat
dc.description.abstractTo determine the effect of aging on IFN-gamma-induced MHC class II antigen expression, we produced bone marrow¿derived macrophages in vitro. In these conditions, we analyzed the effect of aging on the genomic expression of macrophages without the influence of other cell types that may be affected by aging. Although macrophages from young and aged mice showed an identical degree of differentiation, after incubation with IFN-gamma, the expression at the cell surface of the IA complex and the levels of IAbeta protein and mRNA were lower in aged macrophages. Moreover, the transcription of the IAbeta gene was impaired in aged macrophages. The amount of transcription factors that bound to the W and X, but not to the Y, boxes of the IAbeta promoter gene was lower in aged macrophages. Similar levels of CIITA mRNA were found after IFN-gamma treatment of both young and aged macrophages. This shows that neither the initial cascade that starts after the interaction of IFN-gamma with the receptor nor the second signals involved in the expression of CIITA are impaired in aged macrophages. These data indicate that aging is associated with low levels of MHC class II gene induction by IFN-gamma because of impaired transcription.eng
dc.publisherAmerican Society for Clinical Investigationcat
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofJournal of Clinical Investigation, 2001, vol. 107, núm. 4, p.
dc.rights(c) The American Society for Clinical Investigation, 2001cat
dc.sourceArticles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)-
dc.subject.classificationTranscripció genèticacat
dc.subject.classificationRegulació genèticacat
dc.subject.classificationFisiologia cel·lularcat
dc.titleIFN-gamma-dependent transcription of MHC class II IA is impaired in macrophages from aged miceeng
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

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