Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/96505
Title: | Optimization of a liquid chromatography-tandem mass spectrometry method for the quantification of traces of taxanes in a Corylus avellana cell suspension medium |
Author: | Gallego, Ana Jáuregui Pallarés, Olga Moyano Claramunt, Elisabet Palazón Barandela, Javier Casals i Ribes, Isidre Bonfill Baldrich, Ma. Mercedes |
Keywords: | Cromatografia de líquids Espectrometria de masses Liquid chromatography Mass spectrometry |
Issue Date: | 4-Feb-2015 |
Publisher: | Royal Society of Chemistry |
Abstract: | Since the recent discovery of taxol and other taxanes in Corylus avellana, this plant species has attracted interest as a potential new source of these compounds. However, its low taxane content in comparison with Taxus spp. has restricted research to analytical identification or global quantitation. A feasible and sensitive method based on liquid chromatography-tandem mass spectrometry using a triple quadrupole analyzer was developed for the analysis of taxol and four other taxanes in a Corylus avellana cell suspension medium. Taxanes were extracted from the cell culture medium with dichloromethane and analyzed using electrospray ionization and quantified by multiple-reaction monitoring mode. Methanol and matrix-matching calibration curves using docetaxel as the internal standard were analyzed. Linearity was confirmed over the whole calibration range (0.3-2.1 μg mL−1). The inter- and intra-day precision of taxanes ranged from 80% to 120% and the recovery rates were higher than 80%. Limits of detection were between 0.24-38 ng mL−1 and the limits of quantification were between 0.8-125 ng mL−1. The low detection and quantitation values obtained allowed us to detect small quantities of the released taxanes (120 ng mL−1 of B, 151 ng mL−1 of CF and 105 ng mL−1 of T), which correspond to about 0.5 ng mL−1 of each taxane, in the 20 mL Corylus avellana cell suspension culture medium extracted, even at the beginning of the culture. These results were confirmed by high resolution mass spectrometry. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1039/C5RA00803D |
It is part of: | RSC Advances, 2015, vol. 5 , num. 23, p. 17976-17983 |
URI: | http://hdl.handle.net/2445/96505 |
Related resource: | http://dx.doi.org/10.1039/C5RA00803D |
ISSN: | 2046-2069 |
Appears in Collections: | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
646733.pdf | 589.74 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License