Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/96870
Title: | The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues |
Author: | García-Basteiro, Alberto L. Ismail, Mamudo Rafik Carrilho, Carla Ussene, Esperança Castillo, Paola Chitsungo, Dércio Rodríguez, Cristina Lovane, Lucilia Vergara Gómez, Andrea López Varela, Elisa Mandomando, Inácio Lorenzoni, Cesaltina Ordi i Majà, Jaume Menéndez, Clara Bassat Orellana, Quique Martínez Yoldi, Miguel Julián |
Keywords: | Bacteriologia Microbiologia mèdica Malalties infeccioses Bacteriology Medical microbiology Communicable diseases |
Issue Date: | 10-Feb-2016 |
Publisher: | Nature Publishing Group |
Abstract: | The extent to which the Xpert MTB/RIF (Gene Xpert) contributes to tuberculosis (TB) diagnosis in samples other than sputum and cerebrospinal fluid remains uncertain. We aimed to assess the role of Xpert MTB/RIF for detecting M. tuberculosis in post-mortem tissues. We conducted a study among 30 complete diagnostic autopsies (CDA) performed at the Maputo Central Hospital (Mozambique). Lung tissues were screened for TB in all cases. In addition other tissues were tested when compatible lesions were identified in the histological exam. We used in-house real time PCR and LAMP assays to confirm the presence of M. tuberculosis DNA. The diagnosis of tuberculosis at death was established based on microbiological and histopathological results. Eight out of 30 cases (26.7%) were diagnosed of tuberculosis. Xpert had a sensitivity to detect TB in lung tissue of 87.5% (95% CI 47.3-99.7) and a specificity of 95.7% (95% CI: 78.1-99.9). In-house DNA amplification methods and Xpert showed 93.6% concordance for lung tissue and 100% concordance for brain and liver tissues. The final cause of death was attributable to tuberculosis in four cases. Xpert MTB/RIF may represent a valuable, easy-to perform technique for post-mortem TB diagnosis. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1038/srep20703 |
It is part of: | Scientific Reports, 2016, vol. 6, num. 20703 |
URI: | http://hdl.handle.net/2445/96870 |
Related resource: | http://dx.doi.org/10.1038/srep20703 |
ISSN: | 2045-2322 |
Appears in Collections: | Articles publicats en revistes (ISGlobal) Articles publicats en revistes (Fonaments Clínics) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
garcia-basteiro2016_2078.pdf | 286.22 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License