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http://hdl.handle.net/2445/97065
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DC Field | Value | Language |
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dc.contributor.author | Botta, Albert | - |
dc.contributor.author | Martínez Ocaña, Verónica | - |
dc.contributor.author | Mitjans Arnal, Montserrat | - |
dc.contributor.author | Balboa, Elena M. | - |
dc.contributor.author | Conde, Enma | - |
dc.contributor.author | Vinardell Martínez-Hidalgo, Ma. Pilar | - |
dc.date.accessioned | 2016-04-06T16:21:03Z | - |
dc.date.available | 2016-04-06T16:21:03Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.issn | 0887-2333 | - |
dc.identifier.uri | http://hdl.handle.net/2445/97065 | - |
dc.description.abstract | Oxidative stress can damage cellular components including DNA, proteins or lipids, and may cause several skin diseases. To protect from this damage and addressing consumer's appeal to natural products, antioxidants obtained from algal and vegetal extracts are being proposed as antioxidants to be incorporated into formulations. Thus, the development of reliable, quick and economic in vitro methods to study the cytoactivity of these products is a meaningful requirement. A combination of erythrocyte and cell line-based assays was performed on two extracts from Sargassum muticum, one from Ulva lactuca, and one from Castanea sativa. Antioxidant properties were assessed in erythrocytes by the TBARS and AAPH assays, and cytotoxicity and antioxidant cytoprotection were assessed in HaCaT and 3T3 cells by the MTT assay. The extracts showed no antioxidant activity on the TBARS assay, whereas their antioxidant capacity in the AAPH assay was demonstrated. On the cytotoxicity assays, extracts showed low toxicity, with IC50 values higher than 200 µg/mL. C. sativa extract showed the most favourable antioxidant properties on the antioxidant cytoprotection assays; while S. muticum and U. lactuca extracts showed a low antioxidant activity. This battery of methods was useful to characterize the biological antioxidant properties of these natural extracts. | - |
dc.format.extent | 5 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier Ltd | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.tiv.2013.10.004 | - |
dc.relation.ispartof | Toxicology in Vitro, 2014, vol. 28, num. 1, p. 120-124 | - |
dc.relation.uri | http://dx.doi.org/10.1016/j.tiv.2013.10.004 | - |
dc.rights | (c) Elsevier Ltd, 2014 | - |
dc.source | Articles publicats en revistes (Bioquímica i Fisiologia) | - |
dc.subject.classification | Antioxidants | - |
dc.subject.classification | Plantes medicinals | - |
dc.subject.classification | Estrès oxidatiu | - |
dc.subject.classification | Hemòlisi | - |
dc.subject.classification | Toxicologia | - |
dc.subject.classification | Farmacologia | - |
dc.subject.other | Antioxidants | - |
dc.subject.other | Medicinal plants | - |
dc.subject.other | Oxidative stress | - |
dc.subject.other | Hemolysis | - |
dc.subject.other | Toxicology | - |
dc.subject.other | Pharmacology | - |
dc.title | Erythrocytes and cell line-based assays to evaluate the cytoprotective activity of antioxidant components obtained from natural sources | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 629785 | - |
dc.date.updated | 2016-04-06T16:21:08Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) |
Files in This Item:
File | Description | Size | Format | |
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629785.pdf | 673.37 kB | Adobe PDF | View/Open |
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