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http://hdl.handle.net/2445/98051
Title: | Análisis of Benzalkonium chloride by capillary electrophoresis-electrospray-Tandem mass spectrometry |
Author: | Para, B. V. (Bianca Veronica) Núñez Burcio, Oscar Moyano Morcillo, Encarnación Galcerán Huguet, M. Teresa |
Keywords: | Electroforesi capil·lar Capillary electrophoresis |
Issue Date: | 30-May-2006 |
Publisher: | Wiley-VCH |
Abstract: | Conditions for the separation and determination of benzalkonium chloride (BAC) homologues by CE with UV-detection and CE coupled to MS (IT) using electrospray as ionization source were established. The separation was performed using fused-silica capillaries of 50 μm id and 100 mM acetic acid-ammonium acetate buffer solution at pH 4.5 with 80% of ACN as carrier electrolyte. CE-MS coupling parameters were optimized and methanol-10 mM acetic acid (90:10 v/v) was selected as sheath liquid. Detection limits, based on an S/N of 3:1, were calculated, and values between 0.8 and 1.3 mg/L with CE-ESI/MS and around 0.5 mg/L with CE-ESI-MS/MS, using hydrodynamic injection (15 s, 3.5 kPa), were obtained. Good run-to-run and day-to-day precisions on concentration were achieved with RSDs lower than 8%. Quantitative analysis was carried out by the internal standard method and the calibration curves showed good linearities (r2>0.98). The CE-ESI-MS/MS method was successfully applied to the analysis of BAC in different ophthalmic solutions, allowing the direct determination, identification and confirmation of the BAC homologues presented in these samples. |
Note: | Versió postprint del document publicat a: http://dx.doi.org/10.1002/elps.200500716 |
It is part of: | Electrophoresis, 2006, vol. 27, num. 11, p. 2225-2232 |
URI: | http://hdl.handle.net/2445/98051 |
Related resource: | http://dx.doi.org/10.1002/elps.200500716 |
ISSN: | 0173-0835 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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536299.pdf | 4.44 MB | Adobe PDF | View/Open |
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