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Title: Determination of polyphenolic profiles by liquid chromatography-electrospray-tandem mass spectrometry for the authentication of fruit extracts
Author: Puigventós, Lídia
Navarro Reig, Meritxell
Alechaga Silva, Élida
Núñez Burcio, Oscar
Saurina, Javier
Hernández Cassou, Santiago
Puignou i Garcia, Lluís
Keywords: Polifenols
Productes naturals
Química dels aliments
Cromatografia de líquids
Espectrometria de masses
Natural products
Food composition
Liquid chromatography
Mass spectrometry
Issue Date: 5-Nov-2014
Publisher: Springer Verlag
Abstract: Liquid chromatography-electrospray-tandem mass spectrometry (LC-ESI-MS/MS) was applied to the analysis and authentication of fruit-based products and fruit-based pharmaceutical preparations. A Kinetex C18 reversed-phase column under gradient elution with 0.1 % formic acid aqueous solution and methanol mobile phases was used for the simultaneous determination of 26 polyphenols, allowing an acceptable separation in less than 22 min. Instrumental quality parameters such as limits of detection (LOD, values between 12-14 µg/L for 19 of the 26 analyzed polyphenols), linearity (r2 > 0.991), run-to-run and day-to-day precisions (RSD values lower than 9.9 and 13.5 %, respectively), and accuracy (relative errors lower than 8 %) were established. A simple extraction method, consisting of a sample sonication with acetone:water:hydrochloric acid (70:29.9:0.1 v/v/v) and centrifugation, was proposed. Two calibration procedures, external calibration using standards prepared in water and standard addition, were evaluated for polyphenol quantification in several grape and cranberry fruits and processed fruit products. For a 95 % confidence level, no statistical differences were observed between the two calibration methods (p values between 0.06 and 0.95), denoting that external calibration was suitable enough for the quantitative analysis of polyphenols in fruit-based products. The proposed LC-ESI-MS/MS method was then applied to the analysis of polyphenols in 23 grape-based and cranberry-based natural products and pharmaceutical preparations. Polyphenolic concentration data was then analyzed by principal component analysis (PCA) to extract information of the most significant profile data contributing to authentication of natural extracts according to their fruit of origin.
Note: Versió postprint del document publicat a:
It is part of: Analytical and Bioanalytical Chemistry, 2014, vol. 407, p. 597-608
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ISSN: 1618-2642
Appears in Collections:Articles publicats en revistes (Enginyeria Química i Química Analítica)

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