# name
Development of DNA Aptamers Against Plasmodium falciparum Blood Stages Using Cell-Systematic Evolution of Ligands by EXponential Enrichment.

# metadata.dc.description.abstract[].value
"New biomarkers have to be developed in order to increase the performance of current antigen-based malaria rapid diagnosis. Antibody production often involves the use of laboratory animals and is time-consuming and costly, especially when the target is " - ", whose variable antigen expression
                complicates the development of long-lived biomarkers. To
                circumvent these obstacles, we have applied the Systematic
                Evolution of Ligands by EXponential enrichment method to the
                rapid identification of DNA aptamers against " - "-infected red
                blood cells (pRBCs). Five 70 b-long ssDNA sequences, and their
                shorter forms without the flanking PCR primer-binding regions,
                have been identified having a highly specific binding of pRBCs
                versus non-infected erythrocytes. Structural analysis revealed
                G-enriched sequences compatible with the formation of
                G-quadruplexes. The selected aptamers recognized intracellular
                epitopes with apparent " - "s in the " - "M range in both fixed
                and non-fixed saponin-permeabilized pRBCs, improving
                >30-fold the pRBC detection in comparison with aptamers
                raised against " - " lactate dehydrogenase, the gold standard
                antigen for current malaria diagnostic tests. In thin blood
                smears of clinical samples the aptamers reported in this work
                specifically bound all " - " stages versus non-infected
                erythrocytes, and also detected early and late stages of the
                human malaria parasites " - ", " - " and " - . The results are
                discussed in the context of their potential application in
                future malaria diagnostic devices.

# metadata.dc.relation.ispartof[].value
Journal of biomedical nanotechnology, 2020, vol. 16, num. 3, p. 315-334

# metadata.dc.rights[].value
(c) American Scientific Publishers, 2020

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Development of DNA Aptamers Against Plasmodium falciparum Blood Stages Using Cell-Systematic Evolution of Ligands by EXponential Enrichment.