# name
The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis
                in post-mortem tissues

# metadata.dc.description.abstract[].value
The extent to which the Xpert MTB/RIF (Gene Xpert) contributes
                to tuberculosis (TB) diagnosis in samples other than sputum and
                cerebrospinal fluid remains uncertain. We aimed to assess the
                role of Xpert MTB/RIF for detecting M. tuberculosis in
                post-mortem tissues. We conducted a study among 30 complete
                diagnostic autopsies (CDA) performed at the Maputo Central
                Hospital (Mozambique). Lung tissues were screened for TB in all
                cases. In addition other tissues were tested when compatible
                lesions were identified in the histological exam. We used
                in-house real time PCR and LAMP assays to confirm the presence
                of M. tuberculosis DNA. The diagnosis of tuberculosis at death
                was established based on microbiological and histopathological
                results. Eight out of 30 cases (26.7%) were diagnosed of
                tuberculosis. Xpert had a sensitivity to detect TB in lung
                tissue of 87.5% (95% CI 47.3-99.7) and a specificity of 95.7%
                (95% CI: 78.1-99.9). In-house DNA amplification methods and
                Xpert showed 93.6% concordance for lung tissue and 100%
                concordance for brain and liver tissues. The final cause of
                death was attributable to tuberculosis in four cases. Xpert
                MTB/RIF may represent a valuable, easy-to perform technique for
                post-mortem TB diagnosis.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1038/srep20703

# metadata.dc.relation.ispartof[].value
Scientific Reports, 2016, vol. 6, num. 20703

# metadata.dc.source[].value
Articles publicats en revistes (Fonaments Clínics)

# metadata.dc.title[].value
The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis
                in post-mortem tissues