# name
Microsatellite Genotyping of Plasmodium vivax Isolates from Pregnant Women in Four Malaria Endemic Countries

# metadata.dc.description.abstract[].value
Plasmodium vivax is the most widely distributed human parasite
                and the main cause of human malaria outside the African
                continent. However, the knowledge about the genetic variability
                of P. vivax is limited when compared to the information
                available for P. falciparum. We present the results of a study
                aimed at characterizing the genetic structure of P. vivax
                populations obtained from pregnant women from different malaria
                endemic settings. Between June 2008 and October 2011 nearly 2000
                pregnant women were recruited during routine antenatal care at
                each site and followed up until delivery. A capillary blood
                sample from the study participants was collected for genotyping
                at different time points. Seven P. vivax microsatellite markers
                were used for genotypic characterization on a total of 229 P.
                vivax isolates obtained from Brazil, Colombia, India and Papua
                New Guinea. In each population, the number of alleles per locus,
                the expected heterozygosity and the levels of multilocus linkage
                disequilibrium were assessed. The extent of genetic
                differentiation among populations was also estimated. Six
                microsatellite loci on 137 P. falciparum isolates from three
                countries were screened for comparison. The mean value of
                expected heterozygosity per country ranged from 0.839 to 0.874
                for P. vivax and from 0.578 to 0.758 for P. falciparum. P. vivax
                populations were more diverse than those of P. falciparum. In
                some of the studied countries, the diversity of P. vivax
                population was very high compared to the respective level of
                endemicity. The level of inter-population differentiation was
                moderate to high in all P. vivax and P. falciparum populations
                studied.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1371/journal.pone.0152447

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Microsatellite Genotyping of Plasmodium vivax Isolates from Pregnant Women in Four Malaria Endemic Countries