# name
Sugar nucleotide quantification by liquid chromatography tandem
                mass spectrometry reveals a distinct profile in Plasmodium
                falciparum sexual stage parasites

# metadata.dc.description.abstract[].value
The obligate intracellular lifestyle of Plasmodium falciparum
                and the difficulties to obtain sufficient amounts of biological
                material has hampered the study of specific metabolic pathways
                in the malaria parasite. Thus, for example, the pools of sugar
                nucleotides required to fuel glycosylation reactions have never
                been studied in-depth in well-synchronized asexual parasites or
                in other stages of its life cycle. These metabolites are of
                critical importance, especially considering the renewed interest
                in the presence of N -, O - and other glycans in key parasite
                proteins. In this work, we adapted a liquid chromatography
                tandem mass spectrometry method based on the use of porous
                graphitic carbon (PGC) columns and MS-friendly solvents to
                quantify sugar nucleotides in the malaria parasite. We report on
                the thorough quantification of the pools of these metabolites
                throughout the intraerythrocytic cycle of P. falciparum The
                sensitivity of the method enabled for the first time the
                targeted analysis of these glycosylation precursors in
                gametocytes, the parasite sexual stages that are transmissible
                to the mosquito vector.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:            http://dx.doi.org/10.1042/BCJ20161030

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Sugar nucleotide quantification by liquid chromatography tandem
                mass spectrometry reveals a distinct profile in Plasmodium
                falciparum sexual stage parasites