# name
2-picolylamine derivatization for high sensitivity detection of
                abscisic acid in apicomplexan blood-infecting parasites

# metadata.dc.description.abstract[].value
We have developed a new liquid chromatography-electrospray
                ionization tandem mass spectrometry methodology based on
                2-picolylamine derivatization and positive ion mode detection
                for abscisic acid (ABA) identification. The selected reaction
                leads to the formation of an amide derivative which contains a
                highly active pyridyl group. The enhanced ionization allows for
                a 700-fold increase over commonly monitored unmodified ABA,
                which in turn leads to excellent limits of detection and
                quantification values of 0.03 and 0.15ngmL-1, respectively. This
                method has been validated in the highly complex matrix of a red
                blood cell extract. In spite of the high sensitivity achieved,
                ABA could not be detected in Plasmodium falciparum-infected red
                blood cells, suggesting that, if present, it will be found
                either in ultratrace amounts or as brief bursts at defined time
                points within the intraerythrocytic cycle and/or in the form of
                a biosynthetic analogue.

# metadata.dc.relation.isformatof[].value
Versió postprint del document publicat a:
                http://dx.doi.org/10.1016/j.talanta.2017.03.030

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
2-picolylamine derivatization for high sensitivity detection of
                abscisic acid in apicomplexan blood-infecting parasites