# name
Blood-Stage Parasitaemia and Age Determine Plasmodium falciparum
                and P. vivax Gametocytaemia in Papua New Guinea

# metadata.dc.description.abstract[].value
A better understanding of human-to-mosquito transmission is
                crucial to control malaria. In order to assess factors
                associated with gametocyte carriage, 2083 samples were collected
                in a cross-sectional survey in Papua New Guinea. Plasmodium
                species were detected by light microscopy and qPCR and
                gametocytes by detection of pfs25 and pvs25 mRNA transcripts by
                reverse-transcriptase PCR (qRT-PCR). The parasite prevalence by
                PCR was 18.5% for Plasmodium falciparum and 13.0% for P. vivax.
                52.5% of all infections were submicroscopic. Gametocytes were
                detected in 60% of P. falciparum-positive and 51% of P.
                vivax-positive samples. Each 10-fold increase in parasite
                density led to a 1.8-fold and 3.3-fold increase in the odds of
                carrying P. falciparum and P. vivax gametocytes. Thus the
                proportion of gametocyte positive and gametocyte densities was
                highest in young children carrying high asexual parasite
                densities and in symptomatic individuals. Dilution series of
                gametocytes allowed absolute quantification of gametocyte
                densities by qRT-PCR and showed that pvs25 expression is 10-20
                fold lower than pfs25 expression. Between 2006 and 2010 parasite
                prevalence in the study site has decreased by half. 90% of the
                remaining infections were asymptomatic and likely constitute an
                important reservoir of transmission. However, mean gametocyte
                densities were low (approx. 1-2 gametocyte/muL) and it remains
                to be determined to what extent low-density gametocyte positive
                individuals are infective to mosquitos.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1371/journal.pone.0126747

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Blood-Stage Parasitaemia and Age Determine Plasmodium falciparum
                and P. vivax Gametocytaemia in Papua New Guinea