# name
Consistent acidogenic co-fermentation of waste activated sludge and food waste under thermophilic conditions

# metadata.dc.description.abstract[].value
Acidogenic co-fermentation of waste activated sludge (WAS) and food waste (FW) under thermophilic conditions enhances process consistency, while overcoming the problem of acetic acid consumption due to growing methanogens. Two long-term continuous co-fermentation experiments were carried out with a WAS:FW mixture (70:30 % in VS) at organic loading rate of 8 gVS/(L·d). Experiment 1 assessed the impact of temperature (35 °C and 55 °C) and WAS origin (WAS_A and WAS_B) in two collection periods. Experiment 2 evaluated the consistency at 55 °C by testing three WAS origins (WAS_A, WAS_B and WAS_C) in 3 additional collection periods. Experimental results showed that at 55 °C, the solubilisation yield was enhanced compared to 35 °C, although this did not always lead to higher fermentation yield. The fermentation product profile was affected by the operating temperature, with 55 °C promoting the accumulation of acetic and butyric acids. Acetic acid consumption was only detected at 35 °C in fermenters treating WAS_A, whereas it was not observed in fermenters treating WAS_B. This consumption was prevented at 55 °C, as none of the 13 fermenters continuous operation showed acetic acid consumption. Acetic acid consumption was attributed to species midas_s_9557 (genus Methanosarcina), an aceticlastic methanogen, which did not grow under 55 °C. Temperature had a more significant effect on the microbial community structure than WAS origin. Functional redundancy was demonstrated by each fermenter having its own distinct microbial consortium while maintaining constant metabolic functions at 55 °C. Overall, the acidogenic co-fermentation of WAS and FW at 55 °C is regarded as a robust and consistent biotechnology.

# metadata.dc.rights[].value
cc-by-nc-nd (c) Pérez Esteban, Noemí et al., 2025

# metadata.dc.source[].value
Articles publicats en revistes  (Enginyeria Química i Química Analítica)

# metadata.dc.title[].value
Consistent acidogenic co-fermentation of waste activated sludge and food waste under thermophilic conditions