# name
PEGylated PLGA nanoparticles prepared from nano-emulsion templates as versatile platforms to cross blood-brain barrier models

# metadata.dc.description.abstract[].value
PEGylation prevents aggregation and enhances the systemic circulation of nanoparticles (NPs), improving the
delivery of actives to targeted cells. In this study, a conjugation reaction was used to attach polyethylene glycol
(PEG) chains of molecular weights 750 and 5000 Da onto the surface of poly(lactic-co-glycolic acid) (PLGA) NPs
obtained using the phase inversion composition methods, with carbodiimide/N-hydroxysuccinimide (NHS) and
carbodiimide/sulfo-NHS activation reactions. Proton nuclear magnetic resonance indicated a higher degree of
decoration (ca. 44.7 %) when carbodiimide/sulfo-NHS activation and PEG low molecular weight (750 Da) were
used. Short incubation times (2 h at 37 ◦C) in the presence of 10 % fetal bovine serum showed no significant
changes in particle size compared to pristine NPs. After 5 h of incubation, PEGylated NPs exhibited increase size
(101.4 ± 15.3 nm) and polydispersity (0.6 ± 0.01). The presence of PEG chains decorating NPs reduced antioxidant release from NPs to ca. 10 % after 24 h at 37 ◦C following the Korsmeyer–Peppas model and governed by
a Fickian diffusion mechanism. The antioxidant capacity of NPs showed a dose-activity relationship with ca. 60
% inhibition at 0.16 mg mL− 1 NP concentration and an EC50 of 51.7 ± 3.3 μg mL− 1
. Cell culture studies indicated
no cytotoxicity for PLGA and PEGylated NPs up to 0.05 mg mL− 1
. Internalization studies confirmed cellular
uptake into SHSY5Y cells. The impact of PEGylated NPs on blood-brain barrier (BBB) permeabilization was
evaluated in a BBB-on-chip model, showing that PLGA encapsulation and PEGylated NPs, though to a lesser
extent, facilitated crossing and permeabilization through the endothelial layer, demonstrating their potential for
effective brain delivery.

# metadata.dc.relation.ispartof[].value
Journal of Drug Delivery Science and Technology, 2025, vol. 110

# metadata.dc.rights[].value
cc-by-nc (c) López Mitjavila, Joan Josep et al., 2025

# metadata.dc.source[].value
Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)

# metadata.dc.title[].value
PEGylated PLGA nanoparticles prepared from nano-emulsion templates as versatile platforms to cross blood-brain barrier models