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Looking for a needle in a haystack. SARS-CoV-2 variant characterization in sewage

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SARS-CoV-2 variants are emerging worldwide, and monitoring them is key in providing early warnings. Here, we summarize the different analytical approaches currently used to study the dissemination of SARS-CoV-2 variants in wastewater and discuss their advantages and disadvantages. We also provide preliminary results of two sensitive and cost-effective approaches: variant-specific reverse transcription-nested PCR assays and a nonvariant-specific amplicon deep sequencing strategy that targets three key regions of the viral spike protein. Next-generation sequencing approaches enable the simultaneous detection of signature mutations of different variants of concern in a single assay and may be the best option to explore the real picture at a particular time. Targeted PCR approaches focused on specific signature mutations will need continuous updating but are sensitive and cost-effective.

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Current Opinion in Environmental Science & Health, 2021, vol. 24, p. 100308

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Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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Looking for a needle in a haystack. SARS-CoV-2 variant characterization in sewage