# name
Chemical Composition and Cytoprotective Activities of Methanolic Extract of Asplenium adiantum-nigrum L. (Aspleniaceae)

# metadata.dc.description.abstract[].value
Ferns can be a source of polyphenolic compounds, with the fronds being the main parts  described in ethnopharmacological studies. The present study screened polyphenolic phytochemicals  and evaluated in vitro activities of a methanolic extract of Asplenium adiantum-nigrum L. fronds (AAM),  an Aspleniaceae fern collected from the Prades mountains (Tarragona, Spain). Phytocharacterization  by HPLC-MS/MS confirmed that the major flavonoids isolated in AAM are flavanols while the  major phytochemicals are phenol acids, with chlorogenic acid being the most representative one.  Cytotoxicity, cytoprotection, cellular repair activity, and phototoxicity were determined in vitro in the  presence of 0.01, 0.1, and 1 mg/mL of the extract. No cytotoxicity was obtained in any of the cell lines  tested: non-tumoral (3T3 and HaCaT) and tumoral (HeLa, HepG2, and A549) cells. Additionally,  the polyphenolic extract showed greater protective effect against H2O2 in 3T3 than HaCaT cells.  Despite the low total phenolic content of AAM (1405.68 mg phenolic phytochemicals/kg dry extract),  the cytoprotective activity of this extract could be associated with the synergistic antioxidant action  of their polyphenolic profile. In addition, the extract did not present phototoxicity against the  non-cytotoxic 1.8 J/cm2 dose of UVA light in both non-tumoral cell lines.

# metadata.dc.rights[].value
cc-by (c) Farràs Martínez, Adrià et al., 2022

# metadata.dc.source[].value
Articles publicats en revistes (Bioquímica i Fisiologia)

# metadata.dc.title[].value
Chemical Composition and Cytoprotective Activities of Methanolic Extract of Asplenium adiantum-nigrum L. (Aspleniaceae)