# name
Cytoadhesion to gC1qR through Plasmodium falciparum Erythrocyte
                Membrane Protein 1 in Severe Malaria

# metadata.dc.description.abstract[].value
Cytoadhesion of Plasmodium falciparum infected erythrocytes to
                gC1qR has been associated with severe malaria, but the parasite
                ligand involved is currently unknown. To assess if binding to
                gC1qR is mediated through the P. falciparum erythrocyte membrane
                protein 1 (PfEMP1) family, we analyzed by static binding assays
                and qPCR the cytoadhesion and var gene transcriptional profile
                of 86 P. falciparum isolates from Mozambican children with
                severe and uncomplicated malaria, as well as of a P. falciparum
                3D7 line selected for binding to gC1qR (Pf3D7gC1qR). Transcript
                levels of DC8 correlated positively with cytoadhesion to gC1qR
                (rho = 0.287, P = 0.007), were higher in isolates from children
                with severe anemia than with uncomplicated malaria, as well as
                in isolates from Europeans presenting a first episode of malaria
                (n = 21) than Mozambican adults (n = 25), and were associated
                with an increased IgG recognition of infected erythrocytes by
                flow cytometry. Pf3D7gC1qR overexpressed the DC8 type PFD0020c
                (5.3-fold transcript levels relative to Seryl-tRNA-synthetase
                gene) compared to the unselected line (0.001-fold). DBLbeta12
                from PFD0020c bound to gC1qR in ELISA-based binding assays and
                polyclonal antibodies against this domain were able to inhibit
                binding to gC1qR of Pf3D7gC1qR and four Mozambican P. falciparum
                isolates by 50%. Our results show that DC8-type PfEMP1s mediate
                binding to gC1qR through conserved surface epitopes in DBLbeta12
                domain which can be inhibited by strain-transcending functional
                antibodies. This study supports a key role for gC1qR in
                malaria-associated endovascular pathogenesis and suggests the
                feasibility of designing interventions against severe malaria
                targeting this specific interaction.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1371/journal.ppat.1006011

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Cytoadhesion to gC1qR through Plasmodium falciparum Erythrocyte
                Membrane Protein 1 in Severe Malaria