# name
Role of D-fagomine and omega-3 plyunsaturated fatty acids on gut microbia and related metabolic changes in healthy rats and in a model of fat-induced prediabetes

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Universitat de Barcelona. Facultat de Biologia

# metadata.dc.description.abstract[].value
[eng] Modern lifestyles in an increasing number of human societies include consuming an excess of saturated fats  and refined sugars as well as enjoying microbiologically aseptic environments. Maintaining the appropriate distribution of gut microbial populations  (eubiosis) is  emerging as  an attractive approach to prevent ectopic  fat accumulation and insulin resistance (IR), and to avert the progression of diabetes. Functional food components are those that help maintain the normal bodily functions beyond providing energy or building  blocks. They may bring about physiological health benefits for both healthy subjects following  a  healthy lifestyle and people belonging to risk groups such as obese or pre-diabetic populations. D-Fagomine is an iminosugar originally present in buckwheat with the capacity for  selectively inhibiting bacterial adhesion  to the intestinal mucosa. ω-3 Polyunsaturated fatty acids (PUFAs) reduce blood pressure as well as  levels of insulin, triacylglycerols, cholesterol and total lipids. Iminosugars and  ω-3 PUFAs  may  be  used as  functional food ingredients or dietary supplements to maintain a healthy status over time and to reduce risk factors for diabetes.

This thesis focuses on the effects of buckwheat D-fagomine, fish ω-3 PUFAs (EPA/DHA 1:1) and their combination on the gut microbiota and related metabolic variables in the host both in healthy rats and a rat model of fat-induced pre-diabetes.

In the first part of this thesis we explored the effects of D-fagomine  and  ω-3 PUFAs  (EPA/DHA 1:1) on rats given a standard diet as a model for healthy subjects. We found that D-fagomine had the  capacity  for promoting microbial functional diversity by increasing the  Bacteroidetes/Firmicutes ratio and  for mitigating the age-related reduction in populations of the  putatively beneficial Lactobacilliales and Bifidobacteriales. Also, the populations of the genus Prevotella remained stable over time in animals supplemented with D- fagomine, independently of ω-3 PUFAs supplementation. The combination between  D-fagomine  and  ω-3  PUFAs provided the functional benefits of each supplement. Notably, it helped stabilize populations of Prevotella in the rat intestinal tract while reducing weight gain and providing the anti-inflammatory and cardiovascular  benefits of ω-3 PUFAs.

In the second part we explored the effects of the same supplements on rats with fat-induced pre-diabetes as   a model for people at risk of suffering from diabetes and cardiovascular diseases. We found that D-fagomine delayed the development of a fat-induced pre-diabetic state by reducing low-grade inflammation. We suggest that the anti-inflammatory effect of D-fagomine may be linked to a reduction in fat-induced overpopulation of minor gut bacterial groups such as Enterobacteriales. The combined supplements counteracted the high-fat induced incipient IR, and liver inflammation, while increasing the cecal content, the Bacteroidetes/Firmicutes ratio as  well as  the populations  of putatively beneficial     Bifidobacteriales and Lactobacilliales. The functional
 
effects of the combination between D-fagomine and EPA/DHA 1:1 against the gut dysbiosis and the very early metabolic alterations induced by a high-fat westernized diet were mainly those of D-fagomine complemented by the anti-inflammatory action of ω-3 PUFAs.

The results of this thesis point clearly towards a functional role for D-fagomine in the maintenance of the intestinal health by preserving diversity and mitigating the age-related reduction of some beneficial bacteria and also in the prevention of risk factors for diet-induced pre-diabetes reinforced by the action of ω-3 PUFAs  by  complementary mechanisms.

# metadata.dc.title[].value
Role of D-fagomine and omega-3 plyunsaturated fatty acids on gut microbia and related metabolic changes in healthy rats and in a model of fat-induced prediabetes