# name
Evidence for functional pre-coupled complexes of receptor heteromers and adenylyl cyclase

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Canela Campos, Enric I. (Enric Isidre), 1949-

# metadata.dc.description.abstract[].value
G protein-coupled receptors (GPCRs), G proteins and adenylyl cyclase (AC) comprise one of the most studied transmembrane cell signaling pathways. However, it is unknown whether the ligand-dependent interactions between these signaling molecules are based on random collisions or the rearrangement of pre-coupled elements in a macromolecular complex. Furthermore, it remains controversial whether a GPCR homodimer coupled to a single heterotrimeric G protein constitutes a common functional unit. Using a peptide-based approach, we here report evidence for the existence of functional pre-coupled complexes of heteromers of adenosine A2A receptor and dopamine D2 receptor homodimers coupled to their cognate Gs and Gi proteins and to subtype 5 AC. We also demonstrate that this macromolecular complex provides the necessary frame for the canonical Gs-Gi interactions at the AC level, sustaining the ability of a Gi-coupled GPCR to counteract AC activation mediated by a Gs-coupled GPCR.

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Nature Communications, 2018, vol. 9, num. 1, pp.1242

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cc-by (c) Navarro Brugal, Gemma et al., 2018

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Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

# metadata.dc.title[].value
Evidence for functional pre-coupled complexes of receptor heteromers and adenylyl cyclase