# name
Serum-derived exosomes from non-viremic animals previously
                exposed to the porcine respiratory and reproductive virus
                contain antigenic viral proteins

# metadata.dc.description.abstract[].value
PRRSV is the etiological agent of one of the most important
                swine diseases with a significant economic burden worldwide and
                limitations in vaccinology. Exosomes are 30-100 nm vesicles of
                endocytic origin. Remarkably, immunizations with exosomes
                containing antigens from tumors or pathogens are capable of
                eliciting protective immune responses, albeit variably, in
                cancer and infectious diseases. Here we describe the isolation,
                molecular composition and immunogenicity of serum-derived
                exosomes from naive animals, from PRRSV viremic animals and from
                animals previously PRRSV infected but already free of viruses
                (non viremic). Exosomes were isolated through size exclusion
                chromatography and characterized by different methodologies.
                Exosome-enriched fractions from naive and natural infected
                animals contained classical tetraspanin exosomal markers (CD63
                and CD81) and high concentrations of particles in the size-range
                of exosomes as detected by nanoparticle tracking analysis and
                cryo-TEM. NanoLC-MS/MS was used to identify viral antigens
                associated to exosomes. PRRSV-proteins were detected in serum
                samples from only viremic animals and from animals previously
                infected already free of viruses (non-viremic), but not in
                controls. Moreover, immune sera from pigs previously exposed to
                PRRSV specifically reacted against exosomes purified from
                non-viremic pig sera in a dose-dependent manner, a reactivity
                not detected when naive sera was used in the assay. To
                facilitate future studies, a scaling-up process was implemented.
                To the best of our knowledge, this is the first molecular
                characterization of serum-derived exosomes from naive pigs and
                pigs actively or previously infected with PRRSV. The presence of
                antigenic viral proteins in serum-derived exosomes free of
                virus, suggest their use as a novel vaccine approach against
                PRRSV.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1186/s13567-016-0345-x

# metadata.dc.relation.ispartof[].value
Veterinary Research, 2016, vol. 47, num. 59

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Serum-derived exosomes from non-viremic animals previously
                exposed to the porcine respiratory and reproductive virus
                contain antigenic viral proteins