# name
Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum

# metadata.dc.description.abstract[].value
--- - i: - Plasmodium falciparum content: - "Malaria transmission requires that some asexual parasites convert into sexual forms termed gametocytes. The initial stages of sexual development, including sexually committed schizonts and sexual rings, remain poorly haracterized, mainly because they are morphologically identical to their sexual counterparts and only a small subset of parasites undergo sexual development. Here, we describe a system for controlled sexual conversion in the human
                malaria parasite " - ", based on conditional expression of the
                PfAP2-G transcription factor. Using this system, ~90 percent of
                the parasites converted into sexual forms upon induction,
                enabling the characterization of committed and early sexual
                stages without further purification. We characterized sexually
                committed schizonts and sexual rings at the transcriptomic and
                phenotypic levels, which revealed down-regulation of genes
                involved in solute transport upon sexual commitment, among other
                findings. The new inducible lines will facilitate the study of
                early sexual stages at additional levels, including multiomic
                characterization and drug susceptibility assays."

# metadata.dc.publisher[].value
American Association for the Advancement of Science

# metadata.dc.rights[].value
cc by (c) Llorà Batlle, Oriol et al, 2020

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum