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Microscopic, chemical, and molecular-biological investigation of the decayed medieval stained window glasses of two Catalonian churches

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We investigated the decayed historical church window glasses of two Catalonian churches, both under Mediterranean climate. Glass surfaces were studied by scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), and X-ray diffraction (XRD). Their chemical composition was determined by avelength-dispersive spectrometry (WDS) microprobe analysis. The biodiversity was investigated by molecular methods: DNA extraction from glass, amplification by PCR targeting the16S rRNA and ITS regions, and fingerprint analyses by denaturing gradient gel electrophoresis (DGGE). Clone libraries containing either PCR fragments of the bacterial 16S rDNA or the fungal ITS regions were screened by DGGE. Clone inserts were sequenced and compared with the EMBL database.

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Versió postprint del document publicat a: 10.1016/j.ibiod.2012.02.008

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International Biodeterioration and Biodegradation, 2012, p. 1-13

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Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)

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Microscopic, chemical, and molecular-biological investigation of the decayed medieval stained window glasses of two Catalonian churches