# name
DNA Calorimetric Force Spectroscopy at Single Base Pair Resolution

# metadata.dc.description.abstract[].value
DNA hybridization is a fundamental molecular reaction with wide-ranging
applications in biotechnology. The knowledge of the temperature dependence of the thermodynamic parameters of duplex formation is crucial for quantitative  predictions throughout the DNA stability range. It is commonly assumed that enthalpies and entropies are temperature independent, and heat capacity changes ΔCp equal zero. However, it has been known that this assumption is a poor approximation for a long time. Here, we combine single-DNA mechanical unzipping experiments using a temperature jump optical trap with a tailored statistical analysis to derive the ten heat-capacity change parameters of the nearest-neighbor model. Calorimetric force spectroscopy establishes a groundbreaking approach to studying nucleic acids that can be further extended to chemically modified DNA, RNA, and DNA/RNA hybrid structures.

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Nature Communications, 2025, vol. 16, p. 2688

# metadata.dc.rights[].value
cc-by-nc-nd (c) Rissone, Paolo et al., 2025

# metadata.dc.source[].value
Articles publicats en revistes (Física de la Matèria Condensada)

# metadata.dc.title[].value
DNA Calorimetric Force Spectroscopy at Single Base Pair Resolution