# name
Ramial chipped wood amendment drives shifts in soil microbial carbon and phosphorus cycling networks across a depth gradient

# metadata.dc.description.abstract[].value
Soil regeneration depends on microbial processes driving nutrient cycling and organic matter retention. Ramial chipped wood (RCW), a carbon-rich organic amendment, stimulates microbial activity and may promote shortterm soil restoration. This study evaluated the effects of RCW on microbial function, focusing on fungal community structure and genes involved in carbon (C) and phosphorus (P) cycling. A field experiment was conducted with tomato (Solanum lycopersicum) under Mediterranean conditions, comparing surface (0–20 cm) and subsurface (20–25 cm) soils treated with low and high RCW doses (RCW-LD, RCW-HD), compost (CMP) and nitrogen-rich organic pellets as control (CTL), representing standard fertilisation practices.RCW-HD enhanced microbial metabolic capacity, particularly in response to amino acids, sugars, and organic P sources. This response was more pronounced in the subsurface, where RCW also improved microbial access to P pools. Functional gene profile showed that RCW-HD enhanced pathways involved in lignin and lignocellulose degradation (e.g. katG, MAN, glcD), and organic P mobilisation and transport (e.g. phoD, ugpC). In contrast, CMP soils showed a higher abundance of genes linked to methanogenesis (e.g. hdrC2, mttC) and P starvation responses (phoB). Fungal community shifted under RCW, favouring saprotrophic and symbiotic taxa associated with wood decomposition (e.g. Thermothelomyces thermophilus, Linnemannia elongata). These findings underscore the potential of RCW to promote functional microbial homeostasis and support its use as a regenerative amendment in organic Mediterranean farming systems.

# metadata.dc.rights[].value
cc-by-nc-nd (c) Johana González-Coria, et al., 2026

# metadata.dc.source[].value
Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

# metadata.dc.title[].value
Ramial chipped wood amendment drives shifts in soil microbial carbon and phosphorus cycling networks across a depth gradient