# name
In vivo and in vitro characterization of a Plasmodium liver
                stage-specific promoter

# metadata.dc.description.abstract[].value
Little is known about stage-specific gene regulation in
                Plasmodium parasites, in particular the liver stage of
                development. We have previously described in the Plasmodium
                berghei rodent model, a liver stage-specific (lisp2) gene
                promoter region, in vitro. Using a dual luminescence system, we
                now confirm the stage specificity of this promoter region also
                in vivo. Furthermore, by substitution and deletion analyses we
                have extended our in vitro characterization of important
                elements within the promoter region. Importantly, the dual
                luminescence system allows analyzing promoter constructs
                avoiding mouse-consuming cloning procedures of transgenic
                parasites. This makes extensive mutation and deletion studies a
                reasonable approach also in the malaria mouse model.
                Stage-specific expression constructs and parasite lines are
                extremely valuable tools for research on Plasmodium liver stage
                biology. Such reporter lines offer a promising opportunity for
                assessment of liver stage drugs, characterization of genetically
                attenuated parasites and liver stage-specific vaccines both in
                vivo and in vitro, and may be key for the generation of
                inducible systems.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1371/journal.pone.0123473

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
In vivo and in vitro characterization of a Plasmodium liver
                stage-specific promoter