# name
Epigenome-Wide Meta-Analysis of Methylation in Children Related
                to Prenatal NO2 Air Pollution Exposure

# metadata.dc.description.abstract[].value
BACKGROUND: Prenatal exposure to air pollution is considered to
                be associated with adverse effects on child health. This may
                partly be mediated by mechanisms related to DNA methylation.
                OBJECTIVES: We investigated associations between exposure to air
                pollution, using nitrogen dioxide (NO2) as marker, and
                epigenome-wide cord blood DNA methylation. METHODS: We
                meta-analyzed the associations between NO2 exposure at
                residential addresses during pregnancy and cord blood DNA
                methylation (Illumina 450K) in four European and North-American
                studies (n=1,508) with subsequent look-up analyses in children
                aged 4 (n=733) and 8 (n=786) years. Additionally, we applied a
                literature-based candidate approach for antioxidant and
                anti-inflammatory genes. To assess influence of exposure at the
                transcriptomics level, we related mRNA expression in blood cells
                to NO2 exposure in 4- (n=111) and 16-year-olds (n=239). RESULTS:
                We found epigenome-wide significant associations (false
                discovery rate (FDR) p<0.05) between maternal NO2 exposure
                during pregnancy and DNA methylation in newborns for 3 CpG sites
                in mitochondria-related genes: cg12283362 (LONP1), cg24172570
                (3.8 kbp upstream of HIBADH), and cg08973675 (SLC25A28). The
                associations with cg08973675 methylation were also significant
                in the older children. Further analysis of antioxidant and
                anti-inflammatory genes revealed differentially methylated CpGs
                in CAT and TPO in newborns (FDR p<0.05). NO2 exposure at the
                time of biosampling in childhood had significant impact on CAT
                and TPO expression. CONCLUSIONS: NO2 exposure during pregnancy
                was associated with differential offspring DNA methylation in
                mitochondria-related genes. Exposure to NO2 was also linked to
                differential methylation as well as expression of genes involved
                in antioxidant defense pathways.

# metadata.dc.publisher[].value
National Institute of Environmental Health Sciences

# metadata.dc.relation.isformatof[].value
Versió postprint del document publicat a:
                http://dx.doi.org/10.1289/EHP36

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Epigenome-Wide Meta-Analysis of Methylation in Children Related
                to Prenatal NO2 Air Pollution Exposure