# name
DiOleyl-RG and related molecules as transfecting agents to improve efficiency of DNA transfection in hard - to - transfect cells

# metadata.dc.description[].value
Treballs Finals de Grau de Farmàcia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, 2020. Tutor/a: Carlos Julian Ciudad Gómez

# metadata.dc.description.abstract[].value
Cancer and Down Syndrome (DS), among others, are genetic diseases due to mutations
or other alterations in cells’ DNA. PolyPurine Reverse Hoogsteen hairpins (PPRHs) have
proved to be an efficient and specific technology for silencing or editing the genes
involved in cancer mutations. In this research assignment, new transfecting agents in
combination with PPRHs were tested either to improve transfection into hard-to-transfect
cells such as lymphoma B cells using a new cationic liposome called DO-RG (dioleyl-
RG); or to improve the efficiency of this process using gold nanoparticles, which already
have great advantages according to literature. Apart from that, DO-RG was used as a
transfecting vehicle of specific PPRH against DYRK1A, a key protein in DS main
cognitive deficiencies and therefore this treatment would improve DS features. DO-RG
has previously proved to efficiently transfect neuronal origin cells. Promising results are
obtained from the three experiment blocks; however, more replicates should be carried
out to prove statistical significance.

# metadata.dc.source[].value
Treballs Finals de Grau (TFG) - Farmàcia

# metadata.dc.title[].value
DiOleyl-RG and related molecules as transfecting agents to improve efficiency of DNA transfection in hard - to - transfect cells