# name
Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants

# metadata.dc.description.abstract[].value
The enzyme 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase catalyzes the NADPH-mediated reductive deacylation of HMG-CoA to mevalonic acid, which is the first committed step of the mevalonate pathway for isoprenoid biosynthesis. In agreement with its key regulatory role in the pathway, plant HMG-CoA reductase is modulated by many diverse external stimuli and endogenous factors and can be detected to variable levels in every plant tissue. A fine determination of HMG-CoA reductase activity levels is required to understand its contribution to plant development and adaptation to changing environmental conditions. Here, we report a procedure to reliably determine HMG-CoA reductase activity in plants. The method includes the sample collection and homogenization strategies as well as the specific activity determination based on a classical radiochemical assay.

# metadata.dc.relation.ispartof[].value
Methods in Molecular Biology, 2014, vol. 1153, p. 21-40

# metadata.dc.rights[].value
(c) Springer Science + Business Media, 2014

# metadata.dc.source[].value
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

# metadata.dc.title[].value
Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants