# name
Circulating miRNAs, isomiRs and small RNA clusters in human
                plasma and breast milk

# metadata.dc.description.abstract[].value
Circulating small RNAs, including miRNAs but also isomiRs and
                other RNA species, have the potential to be used as non-invasive
                biomarkers for communicable and non-communicable diseases. This
                study aims to characterize and compare small RNA profiles in
                human biofluids. For this purpose, RNA was extracted from plasma
                and breast milk samples from 15 healthy postpartum mothers.
                Small RNA libraries were prepared with the NEBNext(R) small RNA
                library preparation kit and sequenced in an Illumina HiSeq2000
                platform. miRNAs, isomiRs and clusters of small RNAs were
                annotated using seqBuster/seqCluster framework in 5 plasma and
                10 milk samples that passed the initial quality control. The RNA
                yield was 81 ng/mL [standard deviation (SD): 41] and 3985 ng/mL
                (SD: 3767) for plasma and breast milk, respectively. Mean number
                of good quality reads was 4.04 million (M) (40.01% of the reads)
                in plasma and 12.5M (89.6%) in breast milk. One thousand one
                hundred eighty two miRNAs, 12,084 isomiRs and 1,053 small RNA
                clusters that included piwi-interfering RNAs (piRNAs), tRNAs,
                small nucleolar RNAs (snoRNA) and small nuclear RNAs (snRNAs)
                were detected. Samples grouped by biofluid, with 308 miRNAs,
                1,790 isomiRs and 778 small RNA clusters differentially
                detected. In summary, plasma and milk showed a different small
                RNA profile. In both, miRNAs, piRNAs, tRNAs, snRNAs, and snoRNAs
                were identified, confirming the presence of non-miRNA species in
                plasma, and describing them for the first time in milk.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1371/journal.pone.0193527

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Circulating miRNAs, isomiRs and small RNA clusters in human
                plasma and breast milk