# name
High incidence of pandrug-resistant Acinetobacter baumannii
                isolates collected from patients with ventilator-associated
                pneumonia in Greece, Italy and Spain as part of the MagicBullet
                clinical trial

# metadata.dc.description.abstract[].value
Objectives: To investigate the molecular epidemiology,
                antimicrobial susceptibility and carbapenem resistance
                determinants of Acinetobacter baumannii isolates from
                respiratory tract samples of patients diagnosed with
                ventilator-associated pneumonia (VAP) who were enrolled in the
                MagicBullet clinical trial. Methods: A. baumannii isolates were
                prospectively cultured from respiratory tract samples from 65
                patients from 15 hospitals in Greece, Italy and Spain.
                Susceptibility testing was performed by broth microdilution.
                Carbapenem resistance determinants were identified by PCR and
                sequencing. Molecular epidemiology was investigated using
                rep-PCR (DiversiLab) and international clones (IC) were
                identified using our in-house database. Results: Of 65 isolates,
                all but two isolates (97%) were resistant to imipenem and these
                were always associated with an acquired carbapenemase, OXA-23
                (80%), OXA-40 (4.6%), OXA-58 (1.5%) or OXA-23/58 (1.5%).
                Resistance to colistin was 47.7%. Twenty-two isolates were XDR,
                and 20 isolates were pandrug-resistant (PDR). The majority of
                isolates clustered with IC2 ( n = 54) with one major subtype
                comprising isolates from 12 hospitals in the three countries,
                which included 19 XDR and 16 PDR isolates. Conclusions:
                Carbapenem resistance rates were very high in A. baumannii
                recovered from patients with VAP. Almost half of the isolates
                were colistin resistant, and 42 (64.6%) isolates were XDR or
                PDR. Rep-PCR confirmed IC2 is the predominant clonal lineage in
                Europe and suggests the presence of an epidemic XDR/PDR A.
                baumannii clone that has spread in Greece, Italy and Spain.
                These data highlight the difficulty in empirical treatment of
                patients with A. baumannii VAP in centres with a high prevalence
                of carbapenem-resistant A. baumannii .

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1093/jac/dkx322

# metadata.dc.relation.ispartof[].value
The Journal of Antimicrobial Chemotherapy, 2017, vol. 72, num. 12,
                p. 3277–3282

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
High incidence of pandrug-resistant Acinetobacter baumannii
                isolates collected from patients with ventilator-associated
                pneumonia in Greece, Italy and Spain as part of the MagicBullet
                clinical trial