# name
Low frequency of enterohemorrhagic, enteroinvasive and                diffusely adherent Escherichia coli in children under 5 years in                 rural Mozambique: a case-control study

# metadata.dc.description.abstract[].value
BACKGROUND NlmCategory: BACKGROUND content:
                Diarrheagenic Escherichia coli (DEC) are among the leading
                pathogens associated with endemic diarrhea in low income
                countries. Yet, few epidemiological studies have focused the
                contribution of enterohemorrhagic E. coli (EHEC), enteroinvasive
                E. coli (EIEC) and diffusely adherent E. coli (DAEC). - Label:
                METHODS NlmCategory: METHODS content: "We assessed the
                contribution of EHEC, EIEC and DAEC isolated from stool samples
                from a case-control study conducted in children aged
                <\xE2\x80\x895\xE2\x80\x89years in Southern Mozambique
                between December 2007 and November 2012. The isolates were
                screened by conventional PCR targeting stx1 and stx2 (EHEC), ial
                and ipaH (EIEC), and daaE (DAEC) genes." - Label: RESULTS
                NlmCategory: RESULTS content: "We analyzed 297 samples from
                cases with less-severe diarrhea (LSD) matched to 297 controls,
                and 89 samples from cases with moderate-to-severe diarrhea (MSD)
                matched to 222 controls, collected between November 3, 2011 and
                November 2, 2012. DEC were more common among LSD cases (2.7%,
                [8/297] of cases vs. 1.3% [4/297] of controls;
                p\xC2\xA0=\xE2\x80\x890.243]) than in MSD cases (0%, [0/89] of
                cases vs. 0.4%, [1/222]\xC2\xA0of controls;
                p\xC2\xA0=\xE2\x80\x891.000). Detailed analysis revealed low
                frequency of EHEC, DAEC or EIEC and no association with diarrhea
                in all age strata. Although the low frequency, EIEC was
                predominant in LSD cases aged 24-59\xE2\x80\x89months (4.1% for
                cases vs. 0% for controls), followed by DAEC in similar
                frequency for cases and controls in infants (1.9%) and lastly
                EHEC from one control. Analysis of a subset of samples from
                previous period (December 10, 2007 and October 31, 2011) showed
                high frequency of DEC in controls compared to MSD cases (16.2%,
                [25/154] vs. 11.9%, [14/118], p\xC2\xA0=\xE2\x80\x890.383,
                respectively). Among these, DAEC predominated, being detected in
                7.7% of cases vs. 17.6% of controls aged
                24-59\xE2\x80\x89months, followed by EIEC in 7.7% of cases vs.
                5.9% of controls for the same age category, although no
                association was observed. EHEC was detected in one sample from
                cases and two from controls." - Label: CONCLUSIONS NlmCategory:
                CONCLUSIONS content: Our data suggests that although EHEC, DAEC
                and EIEC are less frequent in endemic diarrhea in rural
                Mozambique, attention should be given to their transmission
                dynamics (e.g. the role on sporadic or epidemic diarrhea)
                considering that the role of asymptomatic individuals as source
                of dissemination remains unknown.

# metadata.dc.relation.ispartof[].value
BMC Infectious Diseases, 2020, vol. 20, num. 659

# metadata.dc.rights[].value
cc by (c) Garrine, Marcelino et al., 2020

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Low frequency of enterohemorrhagic, enteroinvasive and                diffusely adherent Escherichia coli in children under 5 years in                 rural Mozambique: a case-control study