# name
Immunostaining Protocol: P-Smad2 (Xenograft and Mice)

# metadata.dc.description.abstract[].value
Metastasis depends on a gene program expressed by the tumor microenvironment
upon TGF-beta stimulation. CRC (Colorectal cancer) cell lines did not induce robust stromal TGF-
beta responses when injected into nude mice as shown by lack of p- SMAD2 accumulation in
tumor-associated stromal cells. To enforce high TGF-beta signaling in xenografts, we engineered
CRC cell lines to secrete active TGF-beta. Subcutaneous tumors obtained from HT29-M6TGF-β,
KM12L4aTGF-β cells and SW48TGF-β cells contained abundant p-SMAD2+ stromal cells.

# metadata.dc.source[].value
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

# metadata.dc.title[].value
Immunostaining Protocol: P-Smad2 (Xenograft and Mice)