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Characterization of thirty two microsatellite loci for three Atlanto-Mediterranean echinoderm species

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Thirty two microsatellites were optimized from 454 pyrosequencing libraries for three Atlanto-Mediterranean echinoderms: Coscinasterias tenuispina, Echinaster sepositus and Arbacia lixula. We observed different frequency of microsatellite types (di-, tri-, tetra- and pentanucleotide) throughout the genome of the species, but no signiﬁcant differences were observed in allele richness among different microsatellite repeats. No loci showed linkage disequilibrium. Heterozygosity deﬁcit and departure from Hardy Weinberg equilibrium were observed for some loci, in two species, probably due to high levels of inbreeding. Heterozygosity excess observed in C. tenuispina could be explained by selection against homozygotes and/or outcrossing.

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Conservation Genetics Resources, 2013, vol. 5, num. 3, p. 749-753

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(c) Springer Science + Business Media, 2013

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Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)

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Characterization of thirty two microsatellite loci for three Atlanto-Mediterranean echinoderm species