# name
Rapid Diagnosis of Staphylococcal Catheter-Related Bacteraemia
                in Direct Blood Samples by Real-Time PCR

# metadata.dc.contributor.author[].value
Martínez, José Antonio (Martínez Martínez)

# metadata.dc.description.abstract[].value
Catheter-related bacteremia (CRB) is an important cause of
                morbidity and mortality among hospitalized patients, being
                staphylococci the main etiologic agents. The objective of this
                study was to assess the use of a PCR-based assay for detection
                of staphylococci directly from blood obtained through the
                catheter to diagnose CRB caused by these microorganisms and to
                perform a cost-effectiveness analysis. A total of 92 patients
                with suspected CRB were included in the study. Samples were
                obtained through the catheter. Paired blood cultures were
                processed by standard culture methods and 4 ml blood samples
                were processed by GeneXpert-MRSA assay for the detection of
                methicillin-susceptible (MSSA) or methicillin-resistant (MRSA)
                Staphylococcus aureus, and methicillin-resistant
                coagulase-negative staphylococci (MR-CoNS). Sixteen CRB caused
                by staphylococci were diagnosed among 92 suspected patients.
                GeneXpert detected 14 out of 16 cases (87.5%), including 4 MSSA
                and 10 MR-CoNS in approximately 1 hour after specimen receipt.
                The sensitivity and specificity of GeneXpert were 87.5% (CI 95%:
                60.4-97.8) and 92.1% (CI 95%: 83-96.7), respectively, compared
                with standard culture methods. The sensitivity of GeneXpert for
                S. aureus was 100%. Regarding a cost-effectiveness analysis, the
                incremental cost of using GeneXpert was of 31.1euro per patient
                while the incremental cost-effectiveness ratio of GeneXpert
                compared with blood culture alones was about 180euro per life
                year gained. In conclusion, GeneXpert can be used directly with
                blood samples obtained through infected catheters to detect S.
                aureus and MR-CoNS in approximately 1h after sampling. In
                addition, it is cost-effective especially in areas with high
                prevalence of staphylococcal CRB.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1371/journal.pone.0161684

# metadata.dc.source[].value
Articles publicats en revistes (Medicina)

# metadata.dc.title[].value
Rapid Diagnosis of Staphylococcal Catheter-Related Bacteraemia
                in Direct Blood Samples by Real-Time PCR