# name
Characterization of vaginal microbiota in women with preterm labor with intra-amniotic inflammation

# metadata.dc.description.abstract[].value
This study aims to investigate the relation between
                vaginal microbiota and exposition to intra-amniotic inflammation
                (IAI). We conducted a prospective cohort study in women with
                preterm labor <34 weeks who had undergone amniocentesis to
                rule out IAI. Vaginal samples were collected after
                amniocentesis. Women with IAI included those with positive
                amniotic fluid (AF) for a microorganism identified by specific
                culture media and Sanger sequencing 16S ribosomal RNA gene
                and/or high AF interleukin (IL)-6 levels. Vaginal microbiota was
                characterized by 16S ribosomal RNA gene amplicon sequencing.
                Specific quantitative PCR targeted to Lactobacillus spp. was
                also performed. Regression models were used to evaluate
                associations between vaginal microbiota and exposition to IAI.
                Concerning our results, 64 women were included. We observed an
                inverse association between AF IL-6 levels and load of
                Lactobacillus spp. Depletion in Lactobacillus spp. load was
                significantly associated with an early gestational age at
                delivery and a short latency to delivery. Microbial-diversity
                was found to be a risk factor for the subsequent occurrence of
                clinical chorioamnionitis. To the contrary, higher Lactobacillus
                spp. load had a protective role. In conclusion, the study
                identifies reduced bacterial load of Lactobacillus spp. in women
                exposed to IAI and found microbial-diversity and Lactobacillus
                spp. depletion to be associated with a worse perinatal outcome.

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Characterization of vaginal microbiota in women with preterm labor with intra-amniotic inflammation