# name
Minimal genetic change in Vibrio cholerae in Mozambique over
                time: Multilocus variable number tandem repeat analysis and
                whole genome sequencing

# metadata.dc.description.abstract[].value
Although cholera is a major public health concern in Mozambique,
                its transmission patterns remain unknown. We surveyed the
                genetic relatedness of 75 Vibrio cholerae isolates from patients
                at Manhica District Hospital between 2002-2012 and 3 isolates
                from river using multilocus variable-number tandem-repeat
                analysis (MLVA) and whole genome sequencing (WGS). MLVA revealed
                22 genotypes in two clonal complexes and four unrelated
                genotypes. WGS revealed i) the presence of recombination, ii) 67
                isolates descended monophyletically from a single source
                connected to Wave 3 of the Seventh Pandemic, and iii) four
                clinical isolates lacking the cholera toxin gene. This Wave 3
                strain persisted for at least eight years in either an
                environmental reservoir or circulating within the human
                population. Our data raises important questions related to where
                these isolates persist and how identical isolates can be
                collected years apart despite our understanding of high change
                rate of MLVA loci and the V. cholerae molecular clock.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1371/journal.pntd.0005671

# metadata.dc.relation.ispartof[].value
PLoS Neglected Tropical Diseases, 2017, vol. 11, num. 6, p.
                e0005671

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Minimal genetic change in Vibrio cholerae in Mozambique over
                time: Multilocus variable number tandem repeat analysis and
                whole genome sequencing