# name
Molecular surveillance of pfhrp2 and pfhrp3 deletions in
                Plasmodium falciparum isolates from Mozambique

# metadata.dc.description.abstract[].value
BACKGROUND: Malaria programmes use Plasmodium falciparum
                histidine-rich protein-2 (PfHRP2) based rapid diagnostic tests
                (RDTs) for malaria diagnosis. The deletion of this target
                antigen could potentially lead to misdiagnosis, delayed
                treatment and continuation of active transmission. METHODS:
                Plasmodium falciparum isolates (n = 1162) collected in Southern
                Mozambique were assessed by RDTs, microscopy and/or 18SrRNA
                qPCR. pfhrp2 and pfhrp3 deletions were investigated in isolates
                from individuals who were negative by RDT but positive by
                microscopy and/or qPCR (n = 69) using gene-specific PCRs, with
                kelch13 PCR as the parasite DNA control. RESULTS: Lack of pfhrp2
                PCR amplification was observed in one of the 69 isolates
                subjected to molecular analysis [1.45% (95% CI 0.3-7.8%)].
                CONCLUSIONS: The low prevalence of pfhrp2 deletions suggests
                that RDTs will detect the vast majority of the P. falciparum
                infections. Nevertheless, active surveillance for changing
                deletion frequencies is required.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1186/s12936-017-2061-z

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Molecular surveillance of pfhrp2 and pfhrp3 deletions in
                Plasmodium falciparum isolates from Mozambique