# name
Characterization of the accessible genome in the human malaria parasite Plasmodium falciparum

# metadata.dc.description.abstract[].value
Human malaria is a devastating disease and a major cause of                 poverty in resource-limited countries. To develop and adapt
                within hosts Plasmodium falciparum undergoes drastic switches in
                gene expression. To identify regulatory regions in the parasite
                genome, we performed genome-wide profiling of chromatin
                accessibility in two culture-adapted isogenic subclones at four
                developmental stages during the intraerythrocytic cycle by using
                the Assay for Transposase-Accessible Chromatin by sequencing
                (ATAC-seq). Tn5 transposase hypersensitivity sites (THSSs)
                localize preferentially at transcriptional start sites (TSSs).
                Chromatin accessibility by ATAC-seq is predictive of active
                transcription and of the levels of histone marks H3K9ac and
                H3K4me3. Our assay allows the identification of novel regulatory
                regions including TSS and enhancer-like elements. We show that
                the dynamics in the accessible chromatin profile matches
                temporal transcription during development. Motif analysis of
                stage-specific ATAC-seq sites predicts the in vivo binding sites
                and function of multiple ApiAP2 transcription factors. At last,
                the alternative expression states of some clonally variant genes
                (CVGs), including eba, phist, var and clag genes, associate with
                a differential ATAC-seq signal at their promoters. Altogether,
                this study identifies genome-wide regulatory regions likely to
                play an essential function in the developmental transitions and
                in CVG expression in P. falciparum.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a: http://dx.doi.org/             10.1093/nar/gky643

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Characterization of the accessible genome in the human malaria parasite Plasmodium falciparum