# name
Assessment of metabolic phenotypic variability in children's
                urine using 1H NMR spectroscopy

# metadata.dc.description.abstract[].value
The application of metabolic phenotyping in clinical and
                epidemiological studies is limited by a poor understanding of
                inter-individual, intra-individual and temporal variability in
                metabolic phenotypes. Using 1H NMR spectroscopy we characterised
                short-term variability in urinary metabolites measured from 20
                children aged 8-9 years old. Daily spot morning, night-time and
                pooled (50:50 morning and night-time) urine samples across six
                days (18 samples per child) were analysed, and 44 metabolites
                quantified. Intraclass correlation coefficients (ICC) and mixed
                effect models were applied to assess the reproducibility and
                biological variance of metabolic phenotypes. Excellent
                analytical reproducibility and precision was demonstrated for
                the 1H NMR spectroscopic platform (median CV 7.2%). Pooled
                samples captured the best inter-individual variability with an
                ICC of 0.40 (median). Trimethylamine, N-acetyl neuraminic acid,
                3-hydroxyisobutyrate, 3-hydroxybutyrate/3-aminoisobutyrate,
                tyrosine, valine and 3-hydroxyisovalerate exhibited the highest
                stability with over 50% of variance specific to the child. The
                pooled sample was shown to capture the most inter-individual
                variance in the metabolic phenotype, which is of importance for
                molecular epidemiology study design. A substantial proportion of
                the variation in the urinary metabolome of children is specific
                to the individual, underlining the potential of such data to
                inform clinical and exposome studies conducted early in life.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1038/srep46082

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Assessment of metabolic phenotypic variability in children's
                urine using 1H NMR spectroscopy